Journal:

Article Title: Preparation and Characterization of Neisseria meningitidis Mutants Deficient in Production of the Human Lactoferrin-Binding Proteins LbpA and LbpB

doi:

Figure Lengend Snippet: Western blot analysis of N. meningitidis mutants. N. meningitidis cells that were grown under iron-limiting conditions to similar optical densities were boiled in Laemmli sample buffer prior to SDS-PAGE and Western blotting. The blots were blocked and incubated with rabbit polyclonal antisera specific for Mbp::LbpB. Binding of the rabbit polyclonal antibodies was detected by using anti-rabbit antibody-HRP conjugate and developed.

Article Snippet: Prevention of nonspecific binding to either Nitro ME-nitrocellulose (Micron Separations, Westboro, Mass.), used for solid-phase binding assays, or to Immobilon P (Millipore, Bedford, Mass.), used for Western blot analysis, was achieved by using blotting-grade nonfat dry milk blocker, whereas color development was performed with HRP development reagent, which was purchased from Bio-Rad (Richmond, Calif.).

Techniques: Western Blot, SDS Page, Incubation, Binding Assay